RED BLOOD CELL GLYCEROLIZATION/DEGLYCEROLIZATION QUALITY CONTROL RECORD

NUMBER__________ DATE_________ WASHED FOR____________
ADDITIONAL QUALITY CONTROL (If performed)

NET WEIGHT: POST-THAW _______ POST-WASH _______ gm WASTE VOL _______ ml TOTAL HGB CONC: POST-THAW _______ POST-WASH _______ WASTE _______ gm/dl
SUPERNATANT HGB: POST-THAW _______ POST-WASH _______ mg%
METHOD: CHEMSTRIP _______ SPECTROPHOTOMETER _______
REFRACTION: POST-THAW _______ (450-550) POST WASH _______ (20-30)
OSMOLALITY: POST-THAW _______ POST-WASH _______ mOsm/kg H20
 EXTRACELLULAR POTASSIUM POST-WASH _______ mEq/L
POST-WASH BACTERIAL CULTURE: AEROBIC _______ ANAEROBIC _______

 
CALCULATIONS:
DENSITY: Post-thaw = 1.1 +[(hct-20)/1000]
Post-wash = 1.0 +[(hct-5)/1000]
 
TOTAL VOLUME = Net weight/density
RBC VOL = Total Vol X hct SUPT VOL = Total vol - RBC vol
 
TOTAL HGB IN UNIT = Total hgb conc X total volume
TOTAL SUPT HGB IN UNIT = supt hgb conc X supt volume
TOTAL CELLULAR HGB IN UNIT = total hgb - total supt hgb
TOTAL HGB IN WASTE = Waste volume X hgb conc in waste
 

FREEZE-THAW RECOVERY ______________ (%) =

Total cell hgb post-thaw
Total hgb post-thaw

 

 

FREEZE-THAW-WASH RECOVERY

 

Pre/Post Hemoglobin: _________ (%)=

Total cell hgb post-wash
Total hgb post-thaw

Waste Hemoglobin: _________ (%) =

Total cell hgb post-wash
Total hgb post-wash + waste hgb

 

COMMENTS ______________________________________________________________

ACCEPTED BY _____________________________________ DATE _________________