SOP 115 - Introduction

INTRODUCTION


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This Standard Operating Procedure describes the procedures to glycerolize and deglycerolize units of human red cells using a manual method in either the 800 ml primary polyvinyl chloride plastic bag system or in a 1000 ml polyvinyl chloride (PVC) plastic bag.

The red blood cells can be collected into any FDA-approved anticoagulant or anticoagulant-preservative system such as CPD, CPDA-1, CP2D/AS3, CPD/AS1, or CPD/AS5. Prior to freezing the whole blood or additive-stored red blood cells are concentrated to prepare a red blood cell concentrate with a hematocrit value of approximately 75 V%. If the blood was not originally collected into the 800 ml plastic bag system, the red blood cell concentrate must be transferred to a standard 1000 ml polyvinylchloride plastic transfer pack using a sterile docking procedure. The red blood cells are glycerolized and the glycerolized red blood cells are concentrated and the supernatant glycerol removed prior to freezing. The glycerolized red cells are washed in the Haemonetics 115. The Haemonetics 115 cell washer is considered an "open" system and the FDA allows for storage of these cells at 4 C for only 24 hours following washing.

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