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SELF-ADMINISTERED
QUIZ TO EVALUATE PROFICIENCY IN OPERATING
THE
HAEMONETICS ACP215 INSTRUMENT
1. What amount of blood must be collected to be processed in the ACP215?
a. 500 ml
of blood
b. 500 ml of blood from a donor with a hematocrit value of less than 46
V%
c. 450 ml of blood from a donor with a hematocrit value of greater than
46 V%
d. all of the above
e. b and c
2. What should the hematocrit value of the red blood cells be at the time of addition of the 6.2 M glycerol solution?
a. 45 V%
b. 75 V%
c. 90 V%
3. How is the hematocrit of the red blood cell concentrate controlled at the time of glycerolization?
a. hematocrit
is measured
b. hematocrit is estimated from the method used to concentrate the red blood
cells prior to removal of the plasma to prepare the red blood cell concentrate
(1615 X g for 4 minutes)
4. What is the anticoagulant used to collect the blood
a. CPD
b. CP2D
c. CPDA-1
d. all of the above
5. What is the longest period of time the red blood cells can be stored as whole blood or as a red blood cell concentrate at 4 C prior to addition of glycerol?
a. 24 hours
b. 3 days
c. 6 days
d. 8 days
6. What is the temperature of the red blood cell concentrate at the time of addition of glycerol solution?
a. 4 C
b. 20-30 C
c. 34-37 C
7. What is the temperature of the glycerol solution at the time of addition to the red blood cell concentrate?
a. 4 C
b. 20-30 C
c. 34-37 C
8. What determines the volume of 6.2 M glycerol solution added to the red blood cell concentrate?
a. hematocrit
of the unit
b. weight of the red blood cell concentrate
c. volume of red blood cells
d. all of the above
9. How are the glycerolized red blood cells concentrated following addition of glycerol?
a. 1248 X g for
10 minutes with brake off
b. 1248 X g for 10 minutes with brake on
c. 1248 X g for 8 minutes with brake off
d. 1248 X g for 8 minutes with brake on
10. What is the hematocrit of the glycerolized red blood cell concentrate at the time of freezing?
a. 45+5
V%
b. 60+5 V%
c. 70+5 V%
d. 80+5 V%
11. The bag containing the glycerolized red blood cells and the cardboard box are labeled as follows:
a. ABO and Rh
b. infectious disease markers
c. expiration date following storage at -80 C
d. all of the above
12. How is the concentration of glycerol assessed following glycerolization?
a. supernatant
osmolality level of 4800-5000 mOsm/kg H20
b. supernatant refraction level of 480-500
c. supernatant glycerol assay of 40-42 W/V
d. all of the above
13. Why is the glycerolized red blood cell concentrate overwrapped with a plastic bag?
a. to avoid wetting
the PVC bag containing the red blood cells in the water bath during the thaw
procedure.
b. to protect the PVC bag during storage in the frozen state
c. a and b
14. Thawing of frozen glycerolized red blood cells can be done using the following:
a. water bath
with a mechanical pump maintained at 42 C for 45 minutes
b. ten-pouch Thermogenesis thaw bath maintained at 36 C for 40 minutes
c. four-pouch Thermogenesis thaw bath maintained at 36 C for 40 minutes
d. all of the above
15. What should the surface temperature of the thawed glycerolized red blood cells be when measured using an infrared laser scanner or a thermometer?
a. 20-22 C
b. 28-30 C
c. 30-34 C
d. 36-38 C
16. Why are thawed glycerolized red cells washed?
a. to reduce
the glycerol level to less than 5%
b. to reduce the glycerol level to less than 2%
c. to reduce the glycerol level to less than 1%
17. Why must the residual glycerol level be reduced?
a. avoid hemolysis
of the red blood cells following transfusion
b. avoid contamination of the red blood cells
c. avoid incompatibility of the red blood cells
d. all of the above
18. What solutions are used to deglycerolize red blood cells?
a. 50 ml of 12%
sodium chloride and 2 liters of 0.9% sodium chloride-0.2 gm% glucose solution
b. 100 ml of 12% sodium chloride and 2 liters of 0.9% sodium chloride-0.2
gm% glucose solution
c. 150 ml of 12% sodium chloride and 2 liters of 0.9% sodium chloride-0.2
gm% glucose solution
19. What is the resuspension media for the deglycerolized red blood cells?
a. AS-1
b. AS-3
c. AS-5
20. What features of the ACP215 permitted the FDA to approve the instrument as a functionally closed procedure to both glycerolize and deglycerolize red blood cells?
a. in-line filtration
of the glycerol solution, 12% sodium chloride solution, 0.9% sodium chloride-0.2
gm% glucose solution, and additive solution
b. sterile connector device to sterilely dock the PVC bag containing the red
blood cell concentrate to the glycerolization disposable set to glycerolize
the red blood cells and sterile docking of the thawed glycerolized red blood
cells to the deglycerolization disposable set to deglycerolize the red blood
cells
c. external seal on the 275 ml blow-molded disposable bowl
d. all of the above
21. What attachment to the ACP215 instrument is critical to automate the procedure to glycerolize and deglycerolize red blood cells?
a. integrally
attached agitator
b. integrally attached printer
c. both of the above
22. What procedure is used to deglycerolize the red blood cells?
a. external dilution
of the thawed glycerolized red blood cells with 12% sodium chloride and 0.9%
sodium chloride-0.2 gm% glucose solution
b. external dilution with 0.9% sodium chloride-0.2gm% glucose solution
c. washing of the glycerolized red blood cells in the 275 ml blow-molded bowl
on 5 occasions by stopping and starting the centrifuge
d. all of the above
23. At the completion of the deglycerolization cycle, what level of hemolysis in the plastic tubing connecting the disposable bowl to the waste bag is acceptable?
a. color comparator
- less than 5
b. color comparator - less than 3
c. color comparator - greater than 5
d. color comparator - greater than 3
24 If the color comparator reading for the level of hemolysis is greater than 5, what should be done?
a. the deglycerolized
unit should be discarded
b. the deglycerolized unit should be sampled using the sterile connector device
and the blood sample spun to assess the supernatant hemoglobin level
c. the color comparator of greater than 5 indicates the presence of intact
red blood cells
d. the color comparator of greater than 5 indicates excessive hemolysis.
e. none of the above
f. all of the above
25. What should the operator do to certify that the unit of glycerolized red blood cells was properly deglycerolized?
a. evaluate the
color of the fluid in the plastic tubing connecting the disposable bowl to
the waste solution 26. How is the optic system of the ACP215 validated to measure
the degree of hemolysis?
a. the optics system is calibrated using filters 27. What is the source of blood that is frozen as provials
with the frozen red blood cells and frozen in a central laboratory?
a. sera obtained from the donor at the time of collection
of the blood 28. The infectious disease marker testing will document the
following testing:
b. evaluate the integrity of the disposable set used to deglycerolize the
red blood cells.
c. document that the color comparator of less than 5 was observed for the
supernatant solution in the plastic
tubing connecting the disposable blow-molded bowl to the waste solution
d. all of the above
b. the optics system is calibrated with plastic tubing containing different
levels of hemolysis
c. the optics system is calibrated with glass tubes containing different levels
of hemolysis
b. plasma obtained from the anticoagulated blood on the day of collection
of the unit of blood
c. plasma obtained from the anticoagulated blood on the day of freezing of
the red blood cells
a. antibodies to HIV 1/2
b. antibodies to HTLV 1/2
c. hepatitis B surface antigen
d. antibody to hepatitis B core antigen
e. antibody to hepatitis C
f. STS
g. nucleic antibody testing (NAT) for hepatitis C and HIV virus
h. none of the above
i. all of the above
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